Ergine and isoergine have the same formula
LSA’s stereochemistry introduces a complication that molecular weight cannot resolve. Ergine and isoergine contain the same atoms but differ in their arrangement at one stereocentre. A study of ergot alkaloids considered these forms alongside other closely related compounds during liquid-chromatographic analysis.
Chromatography and mass spectrometry answer complementary questions: how compounds separate and which ions they produce. A matching mass is useful evidence, but it does not independently establish which stereoisomer is present. In a solution containing related alkaloids, that distinction is part of identifying the constituents accurately.
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